How to perform a comparison study using the FeedCares Solution

This document provides guidelines on how to perform a comparison study of the FeedCares Solution with a tabletop NIR using local feed samples. 

Using NIR technique for feed analysis is a common practice among feed mills and feed analysis laboratories worldwide. The technique has been proved to be suitable for nutrient values analysis and it is acceptable to use when fast and reliable analysis is needed. Nevertheless, the common NIR-based solutions are usually non-mobile and need an experienced analyst to perform the test.

The FeedCares Solution allows for measuring feed ingredients directly on the spot. It combines a light and robust handheld NIR scanner (1400-2500 nm) with a mobile app and connects to Global Feed Database. This service helps to quickly identify the quality of feed materials and optimize the use of feed ingredients. The calibration models are built with global feed database; therefore, it is not limited to any regions or locations.  

For a good comparison study, it is important to consider the differences between laboratory performances, analysts, lab instruments, sample collection procedures, repetition and statistical analysis of the results. This guideline offers you a suggestion on how to perform your own comparison study.

Sample Selection

To make sure that the measurement results take into account the variability, a number of samples should be used. According to ISO 12099, titled "Guidelines for the application of near infrared spectrometry," at minimum 20 samples are needed to determine bias, slope and standard error of prediction (SEP). Therefore, we would like to recommend you perform the study with at least 20 samples.

Ideally, each sample should be accompanied by 5 replicates to assess the reproducibility. However, if the resources are limited, 3 replicates can also give you basic information.

We recommend you use soybeans as a representative sample of raw materials and TMR as a representative of wet feed for the study. However, you can also choose a sample based on your specific interest. Make sure the sample is in good condition or the same condition as you will use them as feed. For example, silages and TMR should be collected fresh, and grains should be new and not moldy.

Analysis Procedure

• Collect 20 individual feed samples and their replicates. To cover variation that might occur on-site, these collected samples can have a variation in term of components, geographic, and sample conditions.
• Use the same set of samples with both our FeedCares solution and your local NIR devices. Follow the instructions of the scanning protocol and procedure of each device carefully. It is advisable to analyze samples with both the FeedCares solution and other NIR device on the same day to minimize the impact of sample variations.
• Extract the results of the report values from both sources and proceed to results evaluation.

When the FeedCares solution is used, before each scan with the Scanner, a calibration scan will be asked. This can be done with open air calibration and while calibration cap. Please check the device scanning surface to ensure it is cleaned properly and no dirt and moisture is interfering with the calibration. If you would like to know more about the calibration, please check this article: Calibration F-series Scanner – SoilCares.

Note the wavelength dissimilarity between the Scanner and your NIR device, therefore differences are expected. After you scan all the samples with both sources, note down the results of your interested parameters and proceed with the evaluation of the results.

For more details information on how to scan the sample, please see this instruction video or read the document "Getting Started with AgroCares Scanner" sent to your email after purchasing the FeedCares solution.

Results Evaluation

The easiest way to compare the difference is to create a spreadsheet with the parameter values (select one) one row per sample and columns for FeedCares solution results, NIR tabletop results, and the difference between the two.

It is recommended to use a statistical analysis to evaluate the results, such as average, %CV, and standard deviation. You can also use simple linear regression by simply plotting the result of each measured parameter. On one axis represent the results of the scanner and your NIR device results on the other axis.

In case you would like to compare the wet chemistry reference methods, here is the list of the methods for feed analysis used to build the database.

For feed and raw materials

For TMR

Please contact us before starting the assessment, so that we can support you with the procedure and guide you through the process. You can reach out to our support team at support@agrocares.com or contact your account manager.